BPC-157 reconstitution protocol

This protocol describes the reconstitution and storage of lyophilized BPC-157 (Body Protection Compound) in standard research workflows. BPC-157 is a synthetic 15-amino-acid compound derived from a fragment of body protection compound found in human gastric juice. Values below are derived from published compound handling literature; experimental design parameters are the responsibility of the qualified investigator.

At a glance

Parameter	Value
Recommended diluent	Bacteriostatic Water (USP, 0.9% benzyl alcohol)
Recommended volume (5 mg vial)	2.0 mL
Final concentration	2.5 mg/mL
Stability — lyophilized	≥24 months at -20 °C, sealed, light-protected
Stability — reconstituted	30 days at 2–8 °C in original vial
pH sensitivity	Most stable at pH 7.0–7.4. Avoid acidic or alkaline diluents.

Procedure

1. Equilibrate. Allow the vial to come to room temperature before reconstitution.
2. Sterile prep. Wipe the stopper with isopropyl. Use a sterile syringe and needle.
3. Inject diluent slowly. Direct the stream along the inner wall of the vial. BPC-157 dissolves readily; do not force the diluent onto the cake.
4. Swirl, don't shake. The cake should dissolve within ~30 seconds.
5. Verify. Solution should be clear and colorless. Mild iridescence under direct light is normal due to the acetate counterion.

Compound notes

BPC-157 is typically supplied as the acetate salt — the small "BPC-157 acetate" detail on the COA matters because the counterion contributes to the reported total mass. A vial labeled "5 mg" of BPC-157 acetate contains slightly less compound mass than 5 mg of the free compound; this is accounted for in HPLC quantitation. Verify by checking the COA's identity confirmation.

The compound is unusually robust compared to many research compounds: it tolerates oral, intranasal, and parenteral administration routes in published preclinical models, though most research uses subcutaneous or intraperitoneal in rodent work. The acetate form is stable in solution longer than many comparable compounds — but freeze-thaw still introduces degradation, so aliquoting remains the recommended long-term storage approach.

Storage

Reconstituted BPC-157 is stable for approximately 30 days at 2–8 °C. Beyond that, aliquot into single-use tubes and freeze at -20 °C or colder. Lyophilized vials store for at least 24 months at -20 °C light-protected.

Notes

This protocol describes reconstitution parameters used in published compound-handling literature. It is not a recommendation for any particular study design or application. For research use only. Not for human or veterinary use.

References

1. Sikiric P, Seiwerth S, Rucman R, et al. Toxicity by NSAIDs. Counteraction by stable gastric pentadecapeptide BPC 157. Curr Pharm Des 2013;19:76–83. PMID: 22950504
2. Chang CH, Tsai WC, Lin MS, et al. The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration. J Appl Physiol 2011;110:774–780. PMID: 21030672