KPV reconstitution protocol
Reconstitution of KPV (Lys-Pro-Val), the α-MSH-derived anti-inflammatory tripeptide studied in IBD and dermal research models.
RECONSTITUTION & RESEARCH PROTOCOLS
Preclinical literature onlyResearch protocol intensities, summarized from published literature. The math is computed for the vial size you pick. Not a dosing recommendation.
Preclinical IBD and dermal-inflammation research (Kannengiesser, Brzoska). No published human RCT data.
Reference research protocols from published peer-reviewed studies. Each card cites its source. This calculator is not a dosing recommendation. For research use only. Selection of any specific protocol is the responsibility of the qualified investigator under appropriate institutional oversight.
This protocol describes the reconstitution and storage of lyophilized KPV (Lys-Pro-Val) in standard research workflows. KPV is the C-terminal tripeptide fragment of α-MSH (residues 11–13) — it retains the anti-inflammatory properties of the parent hormone but without the melanocortin-receptor activation that drives pigmentation effects. Values below reflect published handling literature; study design is the responsibility of the qualified investigator.
At a glance
| Parameter | Value |
|---|---|
| Recommended diluent | Bacteriostatic Water (USP, 0.9% benzyl alcohol) |
| Recommended volume (10 mg vial) | 2.0 mL |
| Final concentration | 5 mg/mL |
| Stability — lyophilized | ≥24 months at -20 °C, sealed, light-protected |
| Stability — reconstituted | 30 days at 2–8 °C in original vial |
| Routes studied | Subcutaneous, intraperitoneal, oral (rodent) |
Procedure
- Equilibrate the vial to room temperature.
- Sterile prep: wipe stopper with isopropyl. Use sterile syringe and needle.
- Inject diluent slowly along the inner wall. KPV dissolves readily — short sequence, no special handling required.
- Swirl gently. Dissolution completes within ~30 seconds.
- Verify: solution should be clear and colorless.
Compound notes
KPV (Lys-Pro-Val) is a 3-amino-acid peptide derived from α-melanocyte-stimulating hormone (α-MSH). The fragment retains the anti-inflammatory activity of α-MSH — mediated through pathways independent of the melanocortin receptors — without the pigmentation effects. Most published research focuses on:
- Anti-inflammatory effects in gut models (colitis, IBD analogs)
- Cytokine modulation in macrophage and dendritic cell culture
- Wound healing and tissue repair models
The peptide is unusually tolerant of administration route — oral bioavailability has been documented in rodent models, which is rare for peptides this small (oral peptides usually degrade in the gut). Plasma half-life is short (<30 minutes), so research protocols typically use daily or twice-daily administration if sustained exposure is needed.
Storage
Reconstituted KPV is stable for approximately 30 days at 2–8 °C. For longer storage, aliquot into sterile single-use tubes and freeze at -20 °C or colder. Lyophilized stability is ≥24 months at -20 °C light-protected.
Notes
This protocol describes reconstitution parameters from published handling literature. It is not a recommendation for any specific research protocol or design. For research use only. Not for human consumption.
References
- Brzoska T, Luger TA, Maaser C, et al. α-Melanocyte-stimulating hormone and related tripeptides: biochemistry, antiinflammatory and protective effects in vitro and in vivo, and future perspectives for the treatment of immune-mediated inflammatory diseases. Endocr Rev 2008;29:581–602. PMID: 18612139
- Kannengiesser K, Maaser C, Heidemann J, et al. Melanocortin-derived tripeptide KPV has anti-inflammatory potential in murine models of inflammatory bowel disease. Inflamm Bowel Dis 2008;14:324–331. PMID: 18092347
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