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BPC-157 reconstitution protocol
Reconstitution and storage for BPC-157 (Body Protection Compound). Particular notes on stability of the peptide at varying pH and the role of acetate counterion.
RECONSTITUTION & RESEARCH PROTOCOLS
Preclinical literature onlyResearch protocol intensities, summarized from published literature. The math is computed for the vial size you pick. Not a dosing recommendation.
Preclinical only. Most published research is rodent (Sikiric group, Chang et al.). No human RCTs.
Reference research protocols from published peer-reviewed studies. Each card cites its source. This calculator is not a dosing recommendation. For research use only. Selection of any specific protocol is the responsibility of the qualified investigator under appropriate institutional oversight.
This protocol describes the reconstitution and storage of lyophilized BPC-157 (Body Protection Compound) in standard research workflows. BPC-157 is a synthetic 15-amino-acid peptide derived from a fragment of body protection compound found in human gastric juice. Values below are derived from published peptide handling literature; experimental design parameters are the responsibility of the qualified investigator.
At a glance
| Parameter | Value |
|---|---|
| Recommended diluent | Bacteriostatic Water (USP, 0.9% benzyl alcohol) |
| Recommended volume (5 mg vial) | 2.0 mL |
| Final concentration | 2.5 mg/mL |
| Stability — lyophilized | ≥24 months at -20 °C, sealed, light-protected |
| Stability — reconstituted | 30 days at 2–8 °C in original vial |
| pH sensitivity | Most stable at pH 7.0–7.4. Avoid acidic or alkaline diluents. |
Procedure
- Equilibrate. Allow the vial to come to room temperature before reconstitution.
- Sterile prep. Wipe the stopper with isopropyl. Use a sterile syringe and needle.
- Inject diluent slowly. Direct the stream along the inner wall of the vial. BPC-157 dissolves readily; do not force the diluent onto the cake.
- Swirl, don't shake. The cake should dissolve within ~30 seconds.
- Verify. Solution should be clear and colorless. Mild iridescence under direct light is normal due to the acetate counterion.
Compound notes
BPC-157 is typically supplied as the acetate salt — the small "BPC-157 acetate" detail on the COA matters because the counterion contributes to the reported total mass. A vial labeled "5 mg" of BPC-157 acetate contains slightly less peptide mass than 5 mg of the free peptide; this is accounted for in HPLC quantitation. Verify by checking the COA's identity confirmation.
The peptide is unusually robust compared to many research peptides: it tolerates oral, intranasal, and parenteral administration routes in published preclinical models, though most research uses subcutaneous or intraperitoneal in rodent work. The acetate form is stable in solution longer than many comparable peptides — but freeze-thaw still introduces degradation, so aliquoting remains the recommended long-term storage approach.
Storage
Reconstituted BPC-157 is stable for approximately 30 days at 2–8 °C. Beyond that, aliquot into single-use tubes and freeze at -20 °C or colder. Lyophilized vials store for at least 24 months at -20 °C light-protected.
Notes
This protocol describes reconstitution parameters used in published peptide-handling literature. It is not a recommendation for any particular study design or application. For research use only. Not for human consumption.
References
- Sikiric P, Seiwerth S, Rucman R, et al. Toxicity by NSAIDs. Counteraction by stable gastric pentadecapeptide BPC 157. Curr Pharm Des 2013;19:76–83. PMID: 22950504
- Chang CH, Tsai WC, Lin MS, et al. The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration. J Appl Physiol 2011;110:774–780. PMID: 21030672
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